In vitro micropropagation of ten different Banana genotypes by using single in vitro culture media

Abstract

A study was carried out to investigate the response to ten different banana genotype viz., Gaint, Panchadum, cv.Rose, Rajapuri, Lalkel, Cheankadai, Raja Balae, Mas, Udhayam and Chinali on Murashige and Skoog (MS) basal medium supplemented with BA (3.0 mg/l) + adenine sulphate (2.0mg/l) for shoot multiplication and in vitro shoot rooting((½ MS + 1.0 mg/l IBA) using shoot-tip culture. Lalkel and Cheankadai banana genotype showed significantly highest shoot multiplication on MS medium supplemented with BA (3.0 mg/l) + adenine sulphate (2.0mg/l) as compare to other genotypes. Better rooting was obtained when the shoots were cultured on ½ MS with 1.0 mg/l IBA for all 10 banana genotypes. Lalkel and Cheankadai banana genotype showed significantly showed highest number of root and root length as compare to other genotype.All the ten banana genotypes had shown better in vitro growth using shoot tip culture.