Production of polyclonal antibodies against the BYMV isolate infecting gladiolus crop naturally and development of polyclonal antibodies based serological diagnostic system

Authors

  • B. R. Singh Molecular Virology Laboratory, National Botanical Research Institute, Lucknow, U.P., India Author
  • Javed Musarrat Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh, U.P., India Author
  • Aminuddin Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh, U.P., India Author

DOI:

https://doi.org/10.46492/IJAI/2017.2.1.2

Keywords:

Gladiolus, Bean Yellow Mosaic Virus, Polyclonal Antibodies, Electron Microscopy

Abstract

In this study a bean yellow mosaic virus isolate infecting gladiolus crop with leaf mosaic and color breaking symptoms on flower was characterized based on mechanical transmission, symptomatology on hosts, and viron particle structure and capsid protein molecular weight determination.  The virus isolate was successfully purified from the propagating host and purified viron particles were used for the production of polyclonal antibodies for the development of a cost effective and sensitive serological diagnostic system. The produced polyclonal antibodies would be useful for the assessment of BYMV infection in gladiolus crop and seed materials.  

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Published

2017-04-26

Issue

Vol. 2 No. 1 (2017): Vol. 2, No. 1, June, 2017

Section

Articles

License

Copyright (c) 2017 Author(s)

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Singh, B. R., Musarrat, J., & Aminuddin. (2017). Production of polyclonal antibodies against the BYMV isolate infecting gladiolus crop naturally and development of polyclonal antibodies based serological diagnostic system . International Journal of Agricultural Invention, 2(1), 5-9. https://doi.org/10.46492/IJAI/2017.2.1.2