Antioxidant and bioactive properties of the various solvent extracts of Kappaphycus alvarezii (Doty) cultivated in the Gulf of Mannar coast

Abstract

The aim of the present study is to evaluate various solvent’s efficiency in extraction (aqueous, 40% ethanol, 60% ethanol, and 80% ethanol) for FTIR, total phenolic content, total flavonoid content, DPPH antioxidant activity, and bioactive properties. The FTIR analysis in the seaweed extracts confirms the presence of polyphenols and flavonoid content and it was predominant in the ethanolic extract. The total phenolic content ranges from 9.87 ± 0.073 -12.82 ± 0.067 mg of GAE.g -1 . Generally, ethanolic extract (E60)
showed enhanced phenolic content, flavonoid content (4.44 ± 0.07 mg of QE.g -1), DPPH assay (IC50 6.50 ± 0.07mg.ml-1
). The best solvent extract (E60) was selected and possesses significant antidiabetic properties as identified by α-Amylase (IC50 0.089 ± 0.01 mg/ml), α-glucosidase (IC50 0.085 ± 0.01 mg/ml), and anti-inflammatory activity (IC50 0.10 ± 0.01 mg /ml). The MTT assay using E60 seaweed extract (0.5 - 2.5mg/ml) conducted against MCF-10A and MCF-7 cells showed maximum inhibition activity of 65.75% and 34.3% respectively. The present findings highlighted that E60 solvent is used for obtaining antioxidant-rich extracts and bioactive properties.